Hoer_carnegie Mellon Open Learning Initiative
last modified by Gillian Stevens (t0022212)
on 15 July 2010 6:26:33 PM
Available Open and Free Courses
http://oli.web.cmu.edu/openlearning/forstudents/freecourses
Biochemistry
http://oli.web.cmu.edu/openlearning/forstudents/freecourses/biochemistry
Overview
- Structural features of proteins and nucleic acids.
- Non-linear (allosteric) behavior of proteins.
- Technological uses of immunogloblins.
- Biochemical catalysts (enzymes).
- Rational design of enzyme inhibitors and drugs.
- Energy generating metabolic pathways and their regulation.
- Production of proteins using modern recombinant DNA techniques.
Content Outline
Introduction
- Functional groups
- Molecular forces
- Water structure and hydrogen bonds
Acids and Buffers
- Acid-base behaviour
- Effect of pH on charge
- Buffers
Protein Structure
- Amino acids
- Structural Hierarchy
- Protein stability
- Case study: Immunoglobulins and Drug detoxification
Binding & Allosteric Effects
- Quantitative analysis of ligand binding
- Case study: Oxygen transport
- Allosteric effects and Cooperative binding
Enzymes
- Catalysts
- Case Study: Serine proteases
- Enzyme kinetics and inhibitors
- Case Study: HIV protease inhibitors
Protein Purification
- Purification methods
- Determination of Quaternary structure
- Structure determination by X-ray diffraction
Carbohydrates
- Mono- and disaccharides
- Polysaccharides
Lipids
- Fatty acids, triglycerides, phospholipids
- Biological membranes
- Case study: Ion channels
Metabolism
- Energetics - Gibbs free energy
- Central pathways in energy generation
- Oxidation of lipids
- Regulation of carbohydrate metabolism
DNA
- Nucleic acid structure and stability
- DNA-protein interactions
- DNA polymerases
- PCR and DNA sequencing
- Properties of expression vectors
- Control of mRNA synthesis in prokaryotes
- Control of protein synthesis in prokayrotes
- Expression of recombinant proteins
- DNA replication