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| h1 __Topics of Interest:__ | h1 __Topics of Interest:__ |
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| h2 __Cell Biology__ | h2 __Cell Biology__ |
| (http://ocw.core.org.cn/CORE/biology/cell-biology/Course_listing) | (http://ocw.core.org.cn/CORE/biology/cell-biology/Course_listing) |
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| __Course Description:__ | __Course Description:__ |
| Cell Biology is to acquaint the undergraduates with the basic knowledge, concepts and fundamental theories of cell structure, function, and the molecular mechanisms of life activities at the level of molecules, sub-cells and cells, as well as the brief history and most advanced fields of the subject being taught. This course include topics: review of cell biology and its researching methods, biomembrane system, genetic information carrier and expression system, cytoskeleton, and cell proliferation, differentiation and cell senescence and apoptosis. All the teaching contents are finished within 51 periods in one semester. | Cell Biology is to acquaint the undergraduates with the basic knowledge, concepts and fundamental theories of cell structure, function, and the molecular mechanisms of life activities at the level of molecules, sub-cells and cells, as well as the brief history and most advanced fields of the subject being taught. This course include topics: review of cell biology and its researching methods, biomembrane system, genetic information carrier and expression system, cytoskeleton, and cell proliferation, differentiation and cell senescence and apoptosis. All the teaching contents are finished within 51 periods in one semester. |
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| __Online Course Contents:__ | __Online Course Contents:__ |
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| __Chapter 1: Introduction__ | Chapter 1: Introduction |
| (http://ocw.core.org.cn/CORE/biology/cell-biology/online-course-1/chapter-1/) | |
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| 1.About Cell Biology | Chapter 2: Techniques in Cell Biology |
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| 2.Look briefly at the history of cell theory | |
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| 3.Why are cells the basic units of life? | |
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| A.The cell is the structural unit of life, All organisms is make up of cells | |
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| B.The cell is the functional unit of organisms. All metabolic activity is based on cells | |
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| C.The cell is the foundation of reproduce, and the bridge of inheritance | |
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| D.The cell is the growing and developing basis of life | |
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| E.Cell (nucleus) is totipotent, which can create a new organism of the same type | |
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| 4.Basic properties of cells | |
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| A.Cells are highly complex and organized, capable of self-regulation;Cells acquire and utilize energy. | |
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| B.All cells share similar structure,composition and metabolic features | |
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| C.Cells can capable of producing more of themselves, even grow and reproduce in culture for extended periods | |
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| D.Cells are able to respond to stimuli via surface receptors that sense changes in the chemical environment | |
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| 5.The Size of Cells | |
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| 6.Two fundamentally different classes of cells: | |
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| Prokaryotes and eukaryotes | |
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| A.Prokaryotic and eukaryotic cells are distinguished by the size and the types of internal structures, or organelles, especially if there is nuclear envelope | |
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| B.Characteristics that distinguish prokaryotic and eukaryotic cells | |
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| C.Three structural systems in Eukaryotes | |
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| 7.Types of Prokaryotic Cells: Two Subkingdoms | |
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| 8.Viruses | |
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| A.Viruses are pathogens first described in the late 1800s | |
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| B.Viral structure | |
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| C.Viral infection types | |
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| D.Viral origins | |
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| __Chapter 2: Techniques in Cell Biology__ | |
| (http://ocw.core.org.cn/CORE/biology/cell-biology/online-course-1/charpter-2/) | |
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| 1.The Light Microscopy | |
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| A.Preparation of specimen | |
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| B.Resolution and magnification | |
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| C.Special Light Microscopes | |
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| 2.Transmission Electron Microscopy | |
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| A.The comparison of the lens systems of LM and TEM | |
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| B.Specimen Preparation for Electron Microscopy | |
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| 3.Scanning electron microscope (SEM) | |
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| 4.The Fractionation and analysis for cell’s contents | |
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| A.The technique of differential centrifugation | |
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| B.Subsequent purification by Density-Gradient Equilibrium Centrifugation | |
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| C.Isolation, purification, and fractionation of proteins | |
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| D.Determining Protein-Protein Interaction | |
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| E.Localization of a specific protein by immuno-electron microscopy | |
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| F.Nucleic acid hybridization | |
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| 5.Protein structure determination | |
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| A.X-ray Crystallography | |
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| B.Nuclear Magnetic Resonance (NMR) Spectroscopy | |
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| 6.The techniques for cytochemistry | |
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| A.Microspectrophotometry | |
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| B.Flow Cytometry | |
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| 7.Autoradiograph of LM and TEM | |
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| 8.The Cell’s cultures and Cell’s engineering | |
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| A.Primary culture cell and subculture cell | |
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| B.Cell strain and Cell line | |
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| C.Cell’s engineering | |
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| D.The technique for the take apart and gather up of cell, and microscope manipulation | |
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| Chapter 3: Biomembranes: Their Structure, Chemistry and Functions | Chapter 3: Biomembranes: Their Structure, Chemistry and Functions |
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| Chapter 4: Extracelldtar matrix, Cell junction and cell adhension | Chapter 4: Extracelldtar matrix, Cell junction and cell adhension |
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| Chapter 5: The Movement of Substances Across Cell Membranes | Chapter 5: The Movement of Substances Across Cell Membranes |
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| Chapter 6: Cell Signaddng | Chapter 6: Cell Signaddng |
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| Chapter 7: Endomembrane system, Protein Sorting and membrane trafficking | Chapter 7: Endomembrane system, Protein Sorting and membrane trafficking |
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| Chapter 8: Energy Generation in Mitochondria and Chloroplasts | Chapter 8: Energy Generation in Mitochondria and Chloroplasts |
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| Chapter 9: Nucleus and Chromosomes | Chapter 9: Nucleus and Chromosomes |
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| Chapter 10: Cytoskeleton System | Chapter 10: Cytoskeleton System |
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| Chapter 11: Cell proddferation and its regdtation | Chapter 11: Cell proddferation and its regdtation |
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| Chapter 12: Cell differentiation and Cancer cells | Chapter 12: Cell differentiation and Cancer cells |
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| Chapter 13: Cell senescence and apoptosis | Chapter 13: Cell senescence and apoptosis |