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| h1 Available Open and Free Courses | h1 Available Open and Free Courses |
| http://oli.web.cmu.edu/openlearning/forstudents/freecourses | http://oli.web.cmu.edu/openlearning/forstudents/freecourses |
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| h1 Biochemistry | h1 Biochemistry |
| http://oli.web.cmu.edu/openlearning/forstudents/freecourses/biochemistry | http://oli.web.cmu.edu/openlearning/forstudents/freecourses/biochemistry |
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| h2 Overview | h2 Overview |
| - Structural features of proteins and nucleic acids. | - Structural features of proteins and nucleic acids. |
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| - Non-linear (allosteric) behavior of proteins. | - Non-linear (allosteric) behavior of proteins. |
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| - Technological uses of immunogloblins. | - Technological uses of immunogloblins. |
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| - Biochemical catalysts (enzymes). | - Biochemical catalysts (enzymes). |
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| - Rational design of enzyme inhibitors and drugs. | - Rational design of enzyme inhibitors and drugs. |
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| - Energy generating metabolic pathways and their regulation. | - Energy generating metabolic pathways and their regulation. |
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| - Production of proteins using modern recombinant DNA techniques. | - Production of proteins using modern recombinant DNA techniques. |
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| | h2 Content Outline |
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| | h3 Introduction |
| | - Functional groups |
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| | - Molecular forces |
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| | - Water structure and hydrogen bonds |
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| | h3 Acids and Buffers |
| | - Acid-base behaviour |
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| | - Effect of pH on charge |
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| | - Buffers |
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| | h3 Protein Structure |
| | - Amino acids |
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| | - Structural Hierarchy |
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| | - Protein stability |
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| | - Case study: Immunoglobulins and Drug detoxification |
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| | h3 Binding & Allosteric Effects |
| | - Quantitative analysis of ligand binding |
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| | - Case study: Oxygen transport |
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| | - Allosteric effects and Cooperative binding |
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| | h3 Enzymes |
| | - Catalysts |
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| | - Case Study: Serine proteases |
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| | - Enzyme kinetics and inhibitors |
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| | - Case Study: HIV protease inhibitors |
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| | h3 Protein Purification |
| | - Purification methods |
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| | - Determination of Quaternary structure |
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| | - Structure determination by X-ray diffraction |
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| | h3 Carbohydrates |
| | - Mono- and disaccharides |
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| | - Polysaccharides |
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| | h3 Lipids |
| | - Fatty acids, triglycerides, phospholipids |
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| | - Biological membranes |
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| | - Case study: Ion channels |
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| | h3 Metabolism |
| | - Energetics - Gibbs free energy |
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| | - Central pathways in energy generation |
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| | - Oxidation of lipids |
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| | - Regulation of carbohydrate metabolism |
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| | h3 DNA |
| | - Nucleic acid structure and stability |
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| | - DNA-protein interactions |
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| | - DNA polymerases |
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| | - PCR and DNA sequencing |
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| | - Properties of expression vectors |
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| | - Control of mRNA synthesis in prokaryotes |
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| | - Control of protein synthesis in prokayrotes |
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| | - Expression of recombinant proteins |
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| | - DNA replication |